The Cloning Procedure for Somatic Cell Nuclear TransferThe Cloning Procedure for Somatic Cell Nuclear Transfer

The Cloning Procedure for Somatic Cell Nuclear Transfer

Somatic Cell Nuclear Transfer

Somatic Cell Nuclear Transfer (SCNT) is a cloning method that involves taking away the nucleus of an ovum and inserting the nucleus from a somatic cell. The nucleus is normally in that case “reprogrammed” by the egg cell possesses the DNA of the donor. After “fusing” the somatic cell's nucleus and ovum, the ovum is stimulated and commences to divide. Following the single cell divides and forms a blastocyst, it really is used in a “surrogate mother”, where in fact the blastocyst will continue steadily to divide and create a nearly similar clone of the somatic cell's donor. The objective of the article was to check the hypothesis that differentiated cells include a higher cloning success amount than adult stem cells.

In order to try out this hypothesis, hematopoietic stem cells at different levels (stem cells, progenitor cells and granulocytes) were used. The first level of somatic cell nuclear transfer, the production of blastocysts, was examined among the stem cells, progenitor cells and granulocytes. In line with the article, the success level of cloning “increased with the level of differentiation, with 8% for stem cells, 11% for progenitor cells, and 35% for the granulocytes. In line with the article, almost all of the stem and progenitor cells

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